Design PCR primers that only amplify your species.
Enter a target species, pick what it shouldn't react with, and get ranked primer pairs — vetted by an AI expert. No account, no cost, just your OpenAI key.
Uses NCBI GenBank · BioPython · 100% client-side API keys.
Engineered for precision
Sequence harvest
Auto-fetches the longest coding region from NCBI GenBank by species and gene — or discovers a marker gene when you leave it blank.
Specificity at the 3' end
Enforces at least two mismatches in the final 5 bases — the exact region where Taq polymerase first fails — so cross-reactions stop before extension.
AI-graded candidates
An AI expert assigns an A / B / C grade and biological rationale to each pair. Template fallback works without a key.
Lab protocol
- 1. Sequence Search
Specify your species
Choose your target organism and what it needs to be distinguished from. Leave the gene blank to let PrimerForge auto-detect a marker gene based on the target's taxonomic group.
- 2. MSA Alignment
Alignment results
Multiple-sequence alignment with species-specific regions highlighted.
- 3. Primer Design
Primer candidates
Candidate pairs that pass every hard filter, listed by genomic position — a screened set for validation, not a ranking.
- 4. AI Report
AI analysis report
The AI generates a narrative brief covering screening criteria, the candidate set, PCR conditions, and cautions.